Extraction in Two-Phase Systems and Some Properties of Laccase from Lentinus polychrous
نویسندگان
چکیده
Extraction of laccase produced by L. polychrous in an aqueous two-phase system, composed of polyethylene glycol and phosphate salt at pH 7.0 and 25C was investigated. The effect of PEG molecular weight, PEG concentration and phosphate concentration was determined. Laccase preferentially partitioned to the top phase. Good extraction of laccase to the top phase was observed with PEG 4000. The optimum system was found in the system containing 12% w/w PEG 4000 and 16% w/w phosphate salt with KE of 88.3, purification factor of 3.0-fold and 99.1% yield. Some properties of the enzyme such as thermal stability, effect of heavy metal ions and kinetic constants were also presented in this work. The thermal stability decreased sharply with high temperature above 60 C. The enzyme was inhibited by Cd, Pb, Zn and Cu. The Vmax and Km values of the enzyme were 74.70 μmol/min/ml and 9.066 mM respectively. Keywords—Aqueous Two Phase System, Laccase, Lentinus polychrous,
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